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Koo2013 Shear-to-NO Composed Pathway

About lab

End-to-end shear-stress driven mechanotransduction. Composes the calcium-influx submodel with the NO-production submodel by wiring the cytosolic calcium output of the first into the cytosolic calcium input of the second. Single experimental knob: stimulus_intensity.

Runtime

Duration600
Comms Step10

Runs

Total3
Completed2
Failed1

Metadata

Packagekoo2013-shear-to-no-composed-pathway
Created2026-05-04
Updated2026-05-14
biomechanicsendothelialshear-stressmechanotransductioncalciumsbmlbiomodels_ebifaithfulnitric-oxidevisualisationkoo2013

Manifest

{
  "io": {
    "inputs": [
      {
        "name": "stimulus_intensity",
        "maps_to": "calcium_influx.stimulus_intensity"
      },
      {
        "name": "integration_step",
        "maps_to": "calcium_influx.integration_step"
      }
    ],
    "outputs": [
      {
        "name": "cytosolic_calcium",
        "maps_to": "calcium_influx.cytosolic_calcium"
      },
      {
        "name": "ip3",
        "maps_to": "calcium_influx.ip3"
      },
      {
        "name": "active_eNOS",
        "maps_to": "no_production.active_eNOS"
      },
      {
        "name": "nitric_oxide",
        "maps_to": "no_production.nitric_oxide"
      },
      {
        "name": "calcium_influx_state",
        "maps_to": "calcium_influx.state"
      },
      {
        "name": "no_production_state",
        "maps_to": "no_production.state"
      }
    ]
  },
  "title": "Koo2013 Shear-to-NO Composed Pathway",
  "models": [
    {
      "path": "owned/models/calcium_influx",
      "alias": "calcium_influx",
      "provenance": {
        "owned_path": "owned/models/calcium_influx"
      }
    },
    {
      "path": "owned/models/no_production",
      "alias": "no_production",
      "provenance": {
        "owned_path": "owned/models/no_production"
      }
    },
    {
      "path": "owned/models/visualisation",
      "alias": "visualisation",
      "provenance": {
        "owned_path": "owned/models/visualisation"
      }
    }
  ],
  "wiring": [
    {
      "to": [
        "no_production.cytosolic_calcium",
        "visualisation.calcium_influx_cytosolic_calcium"
      ],
      "from": "calcium_influx.cytosolic_calcium"
    },
    {
      "to": [
        "visualisation.calcium_influx_ip3"
      ],
      "from": "calcium_influx.ip3"
    },
    {
      "to": [
        "visualisation.no_production_active_eNOS"
      ],
      "from": "no_production.active_eNOS"
    },
    {
      "to": [
        "visualisation.no_production_nitric_oxide"
      ],
      "from": "no_production.nitric_oxide"
    },
    {
      "to": [
        "visualisation.calcium_influx_state"
      ],
      "from": "calcium_influx.state"
    },
    {
      "to": [
        "visualisation.calcium_influx_summary"
      ],
      "from": "calcium_influx.summary"
    },
    {
      "to": [
        "visualisation.no_production_state"
      ],
      "from": "no_production.state"
    },
    {
      "to": [
        "visualisation.no_production_summary"
      ],
      "from": "no_production.summary"
    }
  ],
  "runtime": {
    "duration": 600,
    "initial_inputs": {},
    "communication_step": 10
  },
  "description": "End-to-end shear-stress driven mechanotransduction. Composes the calcium-influx submodel with the NO-production submodel by wiring the cytosolic calcium output of the first into the cytosolic calcium input of the second. Single experimental knob: stimulus_intensity.",
  "schema_version": "2.0"
}

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JSON
{
  "communication_step": 10,
  "dependency_installs": [
    {
      "alias": "calcium_influx",
      "status": "reused"
    },
    {
      "alias": "no_production",
      "reason": "duplicate-lock-hash",
      "status": "skipped"
    }
  ],
  "dependency_policy_enabled": true,
  "duration": 600,
  "lab_commit": null,
  "model_resolutions": [
    {
      "alias": "calcium_influx",
      "commit_sha": null,
      "model_id": "/Volumes/dem-ssd/imp/projects/Nitoons/Biosimulant/models/models-biomechanics/labs/koo2013-shear-to-no/models/calcium-influx",
      "repo_full_name": null
    },
    {
      "alias": "no_production",
      "commit_sha": null,
      "model_id": "/Volumes/dem-ssd/imp/projects/Nitoons/Biosimulant/models/models-biomechanics/labs/koo2013-shear-to-no/models/no-production",
      "repo_full_name": null
    },
    {
      "alias": "visualisation",
      "commit_sha": null,
      "model_id": "/Volumes/dem-ssd/imp/projects/Nitoons/Biosimulant/models/models-biomechanics/labs/koo2013-shear-to-no/models/visualisation",
      "repo_full_name": null
    }
  ],
  "module_ports": {
    "calcium_influx": {
      "inputs": [
        "integration_step",
        "stimulus_intensity"
      ],
      "outputs": [
        "cytosolic_calcium",
        "ip3",
        "state",
        "summary"
      ]
    },
    "no_production": {
      "inputs": [
        "cytosolic_calcium",
        "integration_step"
      ],
      "outputs": [
        "active_eNOS",
        "nitric_oxide",
        "state",
        "summary"
      ]
    },
    "visualisation": {
      "inputs": [
        "calcium_influx_cytosolic_calcium",
        "calcium_influx_ip3",
        "calcium_influx_state",
        "calcium_influx_summary",
        "no_production_active_eNOS",
        "no_production_nitric_oxide",
        "no_production_state",
        "no_production_summary"
      ],
      "outputs": []
    }
  },
  "modules": [
    "calcium_influx",
    "no_production",
    "visualisation"
  ],
  "outputs": {
    "calcium_influx": {
      "cytosolic_calcium": {
        "emitted_at": 600,
        "name": "cytosolic_calcium",
        "source": "calcium_influx",
        "spec": {
          "accepted_profiles": null,
          "description": "Cytosolic Ca2+ amount. Headline downstream readout of shear-stress signalling. Units: substance.",
          "dtype": "float64",
          "emitted_unit": "substance",
          "interpolation": "zoh",
          "kind": "state",
          "max_age": null,
          "schema": null,
          "shape": null,
          "signal_type": "scalar",
          "stale_policy": "warn"
        },
        "type": "scalar",
        "value": 214.82945767646615
      },
      "ip3": {
        "emitted_at": 600,
        "name": "ip3",
        "source": "calcium_influx",
        "spec": {
          "accepted_profiles": null,
          "description": "IP3 (inositol 1,4,5-trisphosphate) amount. Second-messenger driving Ca2+ store release. Units: substance.",
          "dtype": "float64",
          "emitted_unit": "substance",
          "interpolation": "zoh",
          "kind": "state",
          "max_age": null,
          "schema": null,
          "shape": null,
          "signal_type": "scalar",
          "stale_policy": "warn"
        },
        "type": "scalar",
        "value": 26.3950692103414
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      "state": {
        "emitted_at": 600,
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        "source": "calcium_influx",
        "spec": {
          "accepted_profiles": null,
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          "dtype": null,
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          "interpolation": "zoh",
          "kind": "state",
          "max_age": null,
          "schema": {
            "s1": "float",
            "s119": "float",
            "s12": "float",
            "s2": "float",
            "s3": "float",
            "s4": "float",
            "s6": "float"
          },
          "shape": null,
          "signal_type": "record",
          "stale_policy": "warn"
        },
        "type": "record",
        "value": {
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          "s119": 0,
          "s12": 600.0000000000002,
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          "s3": 201.38577538594828,
          "s4": 7548.786789843649,
          "s6": 26.396978990434587
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      },
      "summary": {
        "emitted_at": 600,
        "name": "summary",
        "source": "calcium_influx",
        "spec": {
          "accepted_profiles": null,
          "description": "Final, peak, and minimum value per observable plus simulated duration.",
          "dtype": null,
          "emitted_unit": null,
          "interpolation": "zoh",
          "kind": "state",
          "max_age": null,
          "schema": {
            "duration_simulated": "float",
            "largest_change_magnitude": "float",
            "largest_change_observable": "str",
            "observable_count": "int",
            "peak_observable": "str",
            "peak_value": "float"
          },
          "shape": null,
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        },
        "type": "record",
        "value": {
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    "no_production": {
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        "source": "no_production",
        "spec": {
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          "dtype": "float64",
          "emitted_unit": "substance",
          "interpolation": "zoh",
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          "max_age": null,
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          "signal_type": "scalar",
          "stale_policy": "warn"
        },
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        "value": 0.09277636854945936
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      "nitric_oxide": {
        "emitted_at": 600,
        "name": "nitric_oxide",
        "source": "no_production",
        "spec": {
          "accepted_profiles": null,
          "description": "Nitric oxide (NO) amount. The model's primary downstream readout. Units: substance.",
          "dtype": "float64",
          "emitted_unit": "substance",
          "interpolation": "zoh",
          "kind": "state",
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        "value": 100735.453444921
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      "state": {
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          "dtype": null,
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            "s66": "float"
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        "type": "record",
        "value": {
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          "s45": 0.08805686928343624,
          "s47": 9.541593188758409,
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          "s64": 103080.50248217412,
          "s65": 0.9273922790612495,
          "s66": 0.11821608999029833
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      "summary": {
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        "source": "no_production",
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    "visualisation": {}
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    "visualisation": {}
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          "description": "Which observables shifted the most during the run?",
          "render": "bar"
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            "columns": [
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            "rows": [
              [
                "What did this run track?",
                "7 observables in Koo2013 Shear-Stress Induced Calcium Influx and eNOS Activation"
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              [
                "How long did it run?",
                "590 s"
              ],
              [
                "Which observable changed the most?",
                "Stored calcium"
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              [
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                "Stored calcium"
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            "title": "What Happened"
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            "title": "Koo2013 Shear-Stress Induced NO Production - eNOS activation chain",
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          "render": "timeseries"
        },
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              "Calmodulin Ca4 complex",
              "Hsp90",
              "eNOS-Cav-1",
              "Hsp90-eNOS-CaM-Ca2"
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            "title": "Koo2013 Shear-Stress Induced NO Production - largest excursions during the run",
            "values": [
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          "description": "Which observables shifted the most during the run?",
          "render": "bar"
        },
        {
          "data": {
            "columns": [
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              "Answer"
            ],
            "rows": [
              [
                "What did this run track?",
                "16 observables in Koo2013 Shear-Stress Induced NO Production"
              ],
              [
                "How long did it run?",
                "590 s"
              ],
              [
                "Which observable changed the most?",
                "Nitric oxide"
              ],
              [
                "Largest peak?",
                "Hsp90"
              ]
            ],
            "title": "What Happened"
          },
          "description": "What did this simulation actually show, in plain language?",
          "render": "table"
        }
      ]
    }
  ]
}